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pAL-A3
pAL-A3
規(guī)格:
貨期:
編號:B235932
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pAL-A3
商品貨號 B235932
Designations pAL-A3
Species Unidentified bacterium
Depositors BJ Brown
Applications
produces protein alginate lyase, D-mannuronan specific
Vector
Construct size (kb): 6.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 4.099999904632568
Gene product: alginate lyase, D-mannuronan specific [alxM]
Target Gene: alginate lyase, D-mannuronan specific
Insert Size (kb) 4.100
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.8; PstI/SstI--4.1, 2.7; EcoRI/HindIII--2.7 (doublet), 0.6, 0.45, 0.3, 0.25; KpnI--5.1, 1.7; SalI--3.3, (doublet), 0.25.
The insert contains the following restriction sites (approximate kb from the 5' end): AccI--0.95; AvaI--1.0, 2.8; KpnI--1.6; SalI--0.95.
A periplasmic enzyme preparation of transformed E. coli cells yields 1.7 units per ml of culture broth, compared with 0.4 units per ml of culture broth from the parent organism.
The source strain is a gram-negative marine bacterium associated with the brown algae Sargassum fluitans.
A 1.6 kb KpnI fragment detects the following genomic restriction fragments in the bacterial strain SFFB080483Alg-A (approximate sizes in kb): EcoRI--23; KpnI--1.8; HincII--2 fragments smaller than 1.8.
References

Brown BJ, et al. Cloning of alginate lyase gene (alxM) and expression in Escherichia coli. Appl. Environ. Microbiol. 57: 1870-1872, 1991. PubMed: 1872617

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